Metal Sandwich Method to Quick-freeze Monolayer Cultured Cells for Freeze-fracture
نویسندگان
چکیده
منابع مشابه
Freeze-fracture of monolayer cultures
This paper describes a simple method for the freeze-fracturing of cells in monolayers or multi-layer tissue cultures. The method produces high quality replicas and is applicable to the study of virtually any tissue culture or organ culture system. It uses standard materials and equipment for both tissue culture and freeze-fracturing.
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We have shown that the combination of freeze-fracture with electron microscope autoradiography can be developed into a technique for correlating the molecular structure of the biological membrane with its chemical and functional characteristics. Within the limits of electron microscope autoradiographic resolution, FARG has the potential to detect the relative distribution of molecules in each h...
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densely in the P-face and scattered in the E-face. Like a number of preceding investigators who have failed to obtain convincing findings to correlate similar profiles with the visual pigment molecules, we should also raise a question about preparation involving chemical and thermal procedures affecting the dimensions of the final images. Our constant results obtained from the two methods, howe...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1997
ISSN: 0022-1554,1551-5044
DOI: 10.1177/002215549704500411